Affinity chromatography (IMAC)

Affinity chromatography (IMAC) – His-tagged protein binds Ni²⁺–NTA beads while contaminants flow through; washing, then…

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Affinity chromatography (IMAC): His-tagged protein binds Ni²⁺–NTA beads while contaminants flow through; washing, then imidazole competes for nickel and elutes the pure tagged protein

Affinity chromatography (IMAC): His-tagged protein binds Ni²⁺–NTA beads while contaminants flow through; washing, then imidazole competes for nickel and elutes the pure tagged protein. Flat illustration from the Sciicons Analytical Instruments pack (Pro).

Format: SVG (editable vector). License: Sciicons Pro license. Courtesy: Sciicons. Part of the Analytical Instruments pack.

Keywords: Analytical Instruments Pack, Sciicons Pro, affinity chromatography, IMAC, Ni-NTA, nickel, His tag, histidine tag, imidazole, protein purification, pull-down, analytical chemistry, instrument

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